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Differentiation Cd 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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exosomal marker proteins cd 9  (Proteintech)
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miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
Exosomal Marker Proteins Cd 9, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bv785-conjugated anti-cd-19  (Becton Dickinson)
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miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
Bv785 Conjugated Anti Cd 19, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
Anti Cd 9, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti‑cd‑146  (Proteintech)
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miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
Rabbit Anti‑Cd‑146, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd 146  (Proteintech)
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miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
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miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
J 1500 Cd Spectrometer, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-cd-31 antibodies  (Dawley Inc)
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Dawley Inc anti-cd-31 antibodies
miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of <t>exosomal</t> miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.
Anti Cd 31 Antibodies, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of exosomal miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Gingipains disrupt bone homeostasis via dual regulation of osteogenesis and osteoclastogenesis through exosomal miR-146a-5p/TRAF6 signaling

doi: 10.3389/fcimb.2025.1614126

Figure Lengend Snippet: miR-146a-5p deficiency facilitates the osteoclastic differentiation of RAW264.7 cells in vitro . (A) Heat map diagram of the differential expression of exosomal miRNAs identified by miRNA microarray analysis. (B) Expression of osteoclastic-associated genes ( NFATC1 , CTSK , and TRAP ) in RAW264.7 cells transfected with miR-146a-5p mimics (Mimic group), control mimics (Ctr Mimic group), inhibitor (Inhi group), and control inhibitor (Ctr Inhi group) (n = 6; * P < 0.05). (C) Expression of NFATC1, CTSK, and CD40L/CD154 protein levels in RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi group) (a) . Relative protein expression was quantified by ImageJ software (b) (n = 3; * P < 0.05). (D) TRAP staining of RAW264.7 cells (Mimic, Ctr Mimic, Inhi, and Ctr Inhi groups). * P < 0.05.

Article Snippet: Exosomal marker proteins CD 9 (proteintech, 20597-1-AP, 1:4000) and CD 81 (proteintech, 27855-1- AP, 1:2000) were examined by western blot.

Techniques: In Vitro, Quantitative Proteomics, Microarray, Expressing, Transfection, Control, Software, Staining